Part:BBa_K4161025:Design

pUC19

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 683
  Illegal XbaI site found at 656
  Illegal PstI site found at 644
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 683
  Illegal PstI site found at 644
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 683
  Illegal BamHI site found at 662
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 683
  Illegal XbaI site found at 656
  Illegal PstI site found at 644
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 683
  Illegal XbaI site found at 656
  Illegal PstI site found at 644
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1999
  Illegal SapI.rc site found at 395
  

Design Notes

N/A

Source

Addgene pUC19. https://www.addgene.org/50005/